不同年龄实验大鼠肾脏缺血再灌注的比较研究

Comparative study of renal ischemia-reperfusion between young and aged rats

①目的探讨不同年龄实验大鼠肾缺血再灌注后氧化和抗氧化状态及其机制。②方法SD大鼠24只,随机按鼠龄分为青年大鼠对照组(YCG)、青年大鼠实验组(YMG)、老年大鼠对照组(ACG)、老年大鼠实验组(AMG),每组均为6只。实验组制作肾缺血再灌注模型。分别用硫代巴比妥酸法测定血清MDA含量,亚硝酸盐法检测血清SOD活性。③结果老年大鼠对照组及老年大鼠实验组血清SOD活性显著低于青年大鼠对照组及青年大鼠实验组(P<0.01);老年大鼠实验组血清MDA含量显著高于其对照组(P<0.01)。④结论老年大鼠肾脏缺血再灌注损伤与活性氧引起的氧化损伤以及抗氧化作用的减弱有关;老年大鼠肾脏氧化和抗氧化能力的随增龄变化,其损伤改变更为明显。

Objective To compare the oxidant/antioxidant state in renal ischemia reperfusion injury between young and aged rats and discuss its mechanism. Methods A total of 24 rats were randomly divided into 4 groups （ n = 6） ： young control group （YCG） ,young model group（YMG） ,aged control group（ACG） , and aged model group （AMG）. The renal ischemia reperfusion models were made in experiment group. Contents of malondialdehyde（MDA） and activities of superoxide dismutase （SOD） were determined with thiobarbituric acid reaction and nitriteform method,respectively. Results The SOD activities of blood serum in ACG and AMG were significantly lower than those in YCG and YMG （ P 〈0.01 ）. The MDA content of blood serum in AMG was signifi- cantly higher than that in ACG（ P 〈 0.01 ）. Conclusion The renal ischemia reperfusion injury in aged rats was correlated with both the increased oxidant injury induced by oxide radicals and decreased antioxidant protective action. The renal injury induced by ischemia reperfusion in the aged rats was distinct comparing with the young rats.