生物分子马达动力冲程研究

Power stroke study on molecule motor

通过建立肌球蛋白工作循环的四态模型,利用化学动力学方法和生化热力学原理,计算了动力冲程的大小不仅依赖于动力冲程过程的反应平衡常数及动力冲程前态的分离速率常数,还与肌球蛋白横桥的弹性系数有关。同时,讨论了肌球蛋白横桥的弹性系数与力的关系,为试验研究提供理论依据。

A mechanochemical actin-activated myosin ATPase cycle four-state model had been proposed. Utilizing the chemical kinetic method and biochemical thermodynamic principle, the magnitude of power stroke was calculated. The results showed that it depended on not only the equilibrium constant of the course of the power stroke and the rate at which the first myosin product state can detach from the actin filament, but also on the stiffness of the cross bridge of the myosin. Meanwhile, the relationship between the stiffness of the cross bridge of the myosin and force of muscle contraction was discussed. This results provide theoretical basis for the further experiment.