补肾健脾方对鹅膏蕈氨酸致痴呆大鼠模型学习记忆的影响

Effect of Prescriptions for Reinforcing Kidney and Invigorating Spleen on Memory of Dementia Rat Model Induced by Ibotenic Acid

目的研究中药补肾健脾方对鹅膏蕈氨酸致痴呆大鼠学习记忆功能下降的作用。方法采用鹅膏蕈氨酸(IBO)基底前脑注射造成模型大鼠脑内胆碱能系统损伤,模拟老年性痴呆动物模型。试验分为空白、假手术、模型、小剂量(3.6 g/kg)、大剂量(7.2 g/kg)5组。采用灌胃给药1个月。用水迷宫试验对动物学习记忆能力进行评价。结果模型组大鼠平均逃避潜伏期较空白及假手术组明显延长(P<0.05);大、小剂量组平均逃避潜伏期较模型组明显缩短(P<0.05)。模型组大鼠跨越原平台位置次数较空白及假手术组明显延长(P<0.01);大、小剂量组跨越原平台位置次数较模型组明显缩短(P<0.01)。结论补肾健脾方对鹅膏蕈氨酸致痴呆大鼠的学习记忆能力有增强和提高作用。

OBJECTIVE To investigate into the effect of prescriptions for reinforcing the kidney and invigorating the spleen on the memory of dementia rat model induced by ibotenic acid. METHOD Dementia rat models were made for the study. The subjects were divided into five groups： blank group, pseudo-surgery group, model group, small dosage （3.6 g/kg） group and large dosage （7.2 g/ kg） group. Water maze test was used to test the memory of the experimental animal. RESULT The average escape latent period of the rats in the model group is markedly longer than that of the pseudo-surgery group （ P 〈 0.05） ; the average escape latent period of the large and small dosage groups shortened markedly （ P 〈 0.05）. The number of platform crossing in the model group was markedly greater than that of the blank and pseudo-surgery groups （ P 〈 0.01） and the number of platform crossing in the large and small groups was markedly smaller （ P 〈 0.01 ）. CONCLUSION Prescriptions for reinforcing the kidney and invigorating the spleen can promote the memory of dementia rat model induced by ibotenic acid.