摘要
用芘作为荧光探针,研究了脲素对β-环糊精内腔微环境的影响。结果表明脲素的加入使环糊精内腔极性增大,芘-β-环糊精包络物形成常数减小,这说明“疏水相互作用”是环糊精包络物形成动力之一.这一结论并被其它包络物体系所证实.荧光寿命及荧光量子产率估算表明,脲素的引入不仅仅是改变了溶剂的性质,而可能与β-环糊精分子间有一定的相互作用.
Pyrene was used as a fluorescent probe to illuminate the effect of urea on the microenvironment of β-cycldextrin cavity. Addition of urea to the solution of β-cyclodextrin increased the polarity of β-cyclodextrin cavity and decreased the formation constant of pyrene-β-cyclodextrin inclusion complex, proving that 'hydrophobic interaction' was one of the binding forces of cyclodextrin inclusion complex. The results in other inclusion complex systems strengthened the conclusions. Connected with the results of fluorescence quantum yield and lifetime estimations, a particular interaction of urea with β-cyclodextrin,other than changing the physical properties of the solvent, mainly hydrogen-bonding interaction, was proposed.
