聚肌胞对小鼠的一般毒性和致突变作用

Common toxicity and mutagenic action of poly I∶C in mice

为探讨聚肌胞对实验动物的毒理学效应,本试验通过急性毒性、蓄积毒性、亚慢性毒性和致突变作用等试验研究聚肌胞对昆明系小鼠的一般毒性和特殊毒性。结果表明,聚肌胞注射液对小鼠的LD50为83.5mg·kg-1,95%可信限为66.28-105.32mg·kg-1;聚肌胞注射液对小鼠的蓄积系数（K）大于5,为弱蓄积性,且小鼠对聚肌胞注射液小剂量长期染毒出现耐受现象;亚慢性毒性试验表明,高剂量组死亡小鼠呈现腹泻、肠道臌胀、肠道有水样内容物等消化系统毒性特征的毒性反应,中剂量组呈现微弱毒性反应,而低剂量组无明显毒性;骨髓细胞微核试验、小鼠精子畸形试验结果均为阴性,表明聚肌胞注射液的致突变作用较弱。

To investigate the toxicological effect of poly I∶C（polyinosinic∶polycytidylic acid）on animals,the acute toxicity,accumulative toxicity,inferior-chronic toxicity,mutagenic actions of poly I∶C injection to Kunming mice were studied in this paper.The results revealed that the LD50 of poly I∶C injection in mice was 83.5 mg·kg-1,95% confidence interval was 66.28-105.32 mg·kg-1;poly I∶C injection in mice showed the lower accumulation characteristics with the accumulative coefficient K〉5.The mice showed poly I∶C tolerance with small dosage and long time exposure to poly I∶C injection.The inferior-chronic toxicity of high dose group in mice revealed that poly I∶C injection result in the toxic responses characterized by diarrhea,intestine enlarged with watery contents.Middle dose group mice of poly I∶C injection just showed the lower toxic response and there was no obvious difference in low dose group mice.The negative results of the cellular medullares micronucleus test and the teratospermia test demonstrated that poly I∶C injection had low mutagenic action.