温度预处理对不结球白菜杂种F1花药培养的影响

Effect of temperature pretreatment on the anther culture of F_1 hybrids in non-heading Chinese cabbage

以不结球白菜杂种一代‘99H秋-25’ב99H秋-23’和‘苏州青’בSI’为试材,研究了低温预处理和高温预培养对花药培养效果的影响。结果表明：0-4℃的低温预处理花药1d,花粉可以完成其进行雄核发育所需要的诱导过程,而且绝大部分花粉（93.6%）仍然保持存活,花药分化率达到1.33%;而未经预处理的花药在诱导过程完成时,大多数退化死亡。高温（33℃,24h）处理可明显提高花药再生植株的诱导率。

The effects of low temperature pretreatment and high temperature preculture on the anther culture of non-heading Chinese cabbage were studied,using hybrids＇99H Qiu-25＇×＇99H Qiu-23＇and＇Suzhouqing＇×＇SI＇as material.The results were as follows.Pollen pretreated with 0-4 ℃ low temperature could abort the inducing stage that androgenesis needed,but most pretreated pollen（93.6%）still kept vigor when abortion occurred,and the frequency of callus differentiation reached 1.33%.On the contrary,most unpretreated pollen degenerated and died.And higher temperature pretreatment（33 ℃,24 h）could significantly increase the frequency of plant regeneration.