碳离子辐射大丽花矮化突变体的RAPD分析

RAPD analysis on dwarf mutant of Dahlia pinnata Cav induced by 80 MeV/u^(12) C^(6+) ions

大丽花经兰州重离子加速器提供的80MeV/u12C6+离子束辐照后产生矮化突变体,用随机扩增多态性(Random Amplified Polymorphic,RAPD)DNA技术对野生型和突变体进行检测分析。结果表明,在所用的25条引物中,1.80×108/cm2剂量辐照后有18条引物扩增出现多态性片断,扩增条带多态率19.57%;1.08×108/cm2剂量辐照后仅有6条引物扩增出现多态性片断,扩增条带多态率5.76%。用Jaccard公式对扩增产物进行统计分析,结果表明,两种剂量C6+辐照后与对照相似性系数分别为0.65和0.92。高剂量辐射后DNA易发生突变,在品种改良和诱变育种中相对较高剂量的选择可能更为有效。

Young shoots of Dahlia pinnata Cav cultivar were irradiated by 80 MeV/u 12C6＋ ions. The differences at DNA level between dwarf mutant and wild type were detected by using RAPD （Random amplified polymorphic DNA） method. The results showed that there were different polymorphic bands induced by 18 primers among the tested 25 primers, and the ratio of polymorphic bands and total bands accounted for 19.57% after 1.80×10^8C6＋/cm2 irradiation. Meanwhile, after 1.08×10^8 C6＋/cm2 irradiation the polymorphic bands were induced by only 6 primers and the ratio accounted for 5.76%. Using the Jaccard formula, statistic analysis of amplified bands were obtained and it showed that after 1.80×10^8C6＋/cm2 and 1.08×10^8C6＋/cm2 irradiation the similarity index were 0.65 and 0.92 respec- tively. The results suggested that DNA should prone to mutant at high doses, and high dose irradiation might be more effective in corn improving and mutagenic breeding.