温度对胰高血糖素纤维生长的影响

Temperature effects on growth of glucagon fibers

利用原子力显微镜(Atomic Force Microscopy,AFM)观察了不同温度下胰高血糖素纤维的生长过程,发现在较低温度下(20℃)主要生成粗短的具有螺旋状结构的纤维;在较高温度下(50℃)以细长的非螺旋状纤维为主;而在中间温度下(35℃)这两种类型的纤维数量差不多。统计结果表明,细长的非螺旋状纤维的生长速度比粗短的快很多,说明这两种类型的纤维具有不同结构。同时我们发现,变换生长温度,两种类型的纤维一旦形成能够比较稳定的存在。

Temperature effects on fibrosis growth of glucagon was investigated by atomic force microscope. The protein fibers exhibit different surface structures under various incubation temperatures. At low temperature （20℃）, a majority of fibers take on helix structure. At high temperature （50℃）, the bulk of fibers do not have helix structure and they become a little thinner and much longer than that at low temperature. At midst temperature （35℃）, the amount of the two types of fiber are almost the same, and the thinner fiber grow much faster than the winder one at 35℃. These indicate that the two types of fiber have a different structure. In addition, we found that once the structure is formed, each type fiber was stable at different temperatures.