水中微量芘和荧蒽的胶束增敏荧光分析法同时测定

Simultaneous Determination of Trace Pyrene and Fluoranthene in Water by Micellar-sensitized Fluorescence Spectrophotometry

建立了吐温-20(Tween-20,Tw-20)直接荧光法同时测定水中溶解态芘(pyrene,Py)和荧蒽(fluoran-thene,FLuA)的方法。该法测定芘和荧蒽的线性范围分别为1.98×10-10～9.88×10-8mol/L和2.00×10-9～7.99×10-7mol/L,检出限分别为9.88×10-11mol/L和5.99×10-10mol/L。方法灵敏,用于自来水、江水样的分析,取得了良好的效果,芘的回收率分别为92%～102%和101%～102%,荧蒽的回收率分别为90%～100%和94%～111%。为预测和治理水污染提供了一种灵敏、快速且简便的测试手段。

A direct spectrofluorimetry method was established for simultaneous determination of dissolved pyrene （Py） and fluoranthene（FLuA） in water based on the great enhancement of fluorescence intensities of Py and FLuA in tween-20 （Tw-20） medium. The effects of the types of surfactants, pH, and temperature on the systems were investigated. Under the optimized conditions, the calibration curves showed a good linear range from 1.98 ×10^-10mol to 9. 88 ×10^-8mol/L for Py and 2. 00 ×10^-9 to 7.99 ×10^-7mol/L for FLuA, and their detection limits were 9. 88 ×10^-11mol/L and 5.99 ×10^-10mol/L, respectively. The method was applied in the determination of Py and FLuA in real samples with satisfactory results. The recoveries of pyrene in tap water and river water were 92% - 102% and 101% - 10：2% , and the recoveries of fluoranthene in tap water and river water were 90% - 100% and 94% -111% , respectively. The method is simple, rapid and sensitive for the determination of trace Py and FLuA in the real water sample.