番茄花粉萌发液体培养基的筛选和组分优化

Screening of the Optimal Liquid Culture Medium for Tomato Pollen Germination

以番茄自交系的新鲜混合花粉为材料,采用L16(45)正交试验设计,对番茄花粉的萌发液体培养基进行了筛选和优化。结果表明:浓度配比120 g/L蔗糖,120 mg/L硼酸,4 mg/L赤霉素和0.5 mg/L硫胺素的液体培养基为最佳培养基;硼酸、赤霉素和硫胺素对花粉萌发有显著影响,而蔗糖对花粉萌发没有明显作用。

Orthogonal experimental design of L16 （42 ） was adopted to screen the optimal liquid culture medium according to the pollen germination rate. The influence of cane sugar, boric acid, gibberellic acid to pollen germination was analyzed. The result showed that the optimal liquid culture medium was cane sugar 120 g/L, boric acid 120 mg/L, gibberellic acid 40 mg/L and torulin 0.5 mg/L. Gibberellic acid, torulin and boric acid had much more obvious influence than cane sugar on pollen germination.