多因子正交试验对鞭炮花丛生芽诱导条件的筛选

Selection of Caespitose Shoots Inducing Condition of Crossandra pungens Using Orthogonal Experimental Designed

利用正交试验设计方法探讨6-BA、NAA、2,4-D和蔗糖对丛生芽诱导影响。结果表明:蔗糖和6-BA的浓度对鞭炮花丛生芽诱导的影响最大,高浓度的2,4-D对于愈伤组织的诱导和增殖具有良好的促进作用,但对芽的分化有抑制作用。筛选出诱导鞭炮花丛生芽的最适培养基为MS+2.0 mg/L 6-BA+1.4 mg/L NAA+25 g/L蔗糖+0.7%琼脂。该培养基可使外植体一步诱导出苗,并获得移栽成活的植株。

The effect of plant growth regulators including 6-BA, NAA,2,4-D and sucrose on inducing caespitose shoots of Crossandra pungens was studied using an orthogonal experimental designed. The results showed that the most important factor affecting on inducing caespitose shoots was the ratio of 6-BA and sucrose, high 2,4-D concentration was helpful for propagation of callus and was unfavourable for differentiation of shoot. The best medium inducing caespitose shoots of Crossandra pungens was MS＋6-BA 2. 0 rng/L＋NAA 1.4 mg/L＋sucrose 25.0 g/L. The Seedling could be directly induced from the explant using this medium. And many regeneration plantlets were obtained.