黑木耳蛋白质中硒的分布规律研究

Study on the Selenium Rule in the Soluble Proteins of Auricularia auricula from the High-Se Cultivation

采用补硒培养的富硒黑木耳,经粉碎、脱脂,提取可溶性蛋白质,并通过分成A、B、C、D、E 5组进行分级盐析,用石墨炉原子吸收光度法测定它们各组分中的含硒量。初步研究了黑木耳蛋白质中硒的分布规律。结果表明：在各盐析组分中均有含硒蛋白质存在,以D组硒含量最高,其分布次序为D〉E〉C〉B〉A。因此,组分D（70%-80%硫酸铵饱和度）是最适合开发成为补硒蛋白质食品或添加剂。

This article primarily studied the distribution rule of selenium in the soluble proteins of Auricularia auricula from the high-Se cultivation. The soluble proteins abstracted from Auricularia auricula through adding selenium cultiva- tion were classed to five groups of A,B,C,D,and E through grading salting out, and used atomic absorption spectrometer to determine the selenium content of all component parts. Results showed there were Se-protein in every group protein by salted out. The content of selenium was the highest in D, the quality fraction of the selenium was D〉E〉C〉B〉A. The group of D （The degree of saturation of ammonium sulfate was 70%～80%） was the fittest one for food addiitive.