大花蕙兰快速繁殖技术的研究

Study on Rapid Multiplication Techniques for Cymbidium Hybridism

本文对大花蕙兰外植体消毒方式,原球茎增殖的培养基,大花蕙兰无根菌诱导生根培养基三个方面进行了系统的研究。结果表明:外植体在用加入1%Tween-60的1%HgCl2溶液消毒8分钟效果最明显,利于原球茎增殖的培养基为MS+6-BA1.0mg/L+NAA0.5mg/L,增殖系数为3.97,最佳的生根培养基为MS+NAA0.2mg/l+GA1.0mg/l+香蕉泥150 g/L,生根率最高可以达到100%。

In this paper, several crucial technical questions on the multiplication of Cymbidium Hybridism have been studied, including the method of disinfection ,the best culture medium for multiplication of protocorm like-body （PLB）,and the medium of rooting. The results are as follows： 1%HgCl2 solution with added 1%Tween-60 which has been treated for 8 minutes is most suitable for explants disinfection; The best medium for the propagation of PLB is MS＋6-BA 1.0mg/L＋NAA 0.5 mg/L, the propagation coefficient is 3.97, MS＋NAA0.2mg/1 ＋GA1.0mg/1 ＋ banana mud150g/L is better for the plantlet growth and rooting, and 100%of plantlets rooted well.