血细胞生物参考区间适用性验证探讨

Study on the validation for biological reference interval of blood cells

目的对实验室检测项目白细胞、红细胞、血红蛋白、血小板现用的生物参考区间进行验证,以保证其适用于临床。方法按美国临床实验室标准化委员会C28-A2推荐的方法,各验证项目选择健康参考个体20例,按实验室操作手册要求进行标本采集和检测,采用性能评价符合要求的LH750血细胞分析仪进行检测。结果参与验证的4个项目中,20例健康参考个体结果100%落在现用的生物参考区间内。结论验证项目现用的生物参考区间适用于江苏省中医院实验室,可以继续使用。实验验证具有科学的说服力,容易发现生物参考区间的偏离,值得建立完善的验证制度和程序,并推广应用。

Objective To validate the current biological reference interval of WBC, RBC, HGB and PLT, and ensure their clinical application. Methods According to NCCLS C28-A2 recommended method, 20 healthy individuals were chosen. The whole blood samples were collected and detected by Beckman Coulter LH 750 hematology analyzer according to the laboratory operating manual. Results As for 4 verified projects, all results of 20 reference samples were within the current biological reference interval. Conclusion The current biological reference intervel is suitable for our laboratory and can be still used; the experimental testing has the scientific persuasiveness. It is easy to discover the deviation of the biological reference interval and is necessary to establish a perfect verification system and promote its popularization.