双波长梯度洗脱法测定栀子柏皮汤制剂中栀子苷、甘草苷的含量

Determination of geniposide and liquiritin in Zhizi Baipi Decoction by HPLC

目的:研究以HPLC法同时测定栀子柏皮汤制剂(栀子、黄柏、甘草)中栀子苷、甘草苷含量的方法。方法:采用Kro-masil C18柱(4.6 mm×250 mm,5μm),以甲醇-0.5%冰乙酸为流动相,梯度洗脱,体积流量:1.0 mL/min,λ1=238 nm(检栀子苷),λ2=276 nm(检甘草苷),柱温:30℃。结果:栀子苷在49.1μg/mL^147.4μg/mL与峰面积呈良好的线性关系,r=0.999 8,甘草苷在6.1~18.4μg/mL与峰面积呈良好的线性关系,r=0.999 9。平均加样回收率(n=9):栀子苷为99.40%;甘草苷为99.94%。结论:本法操作简便,结果可靠,重现性好,可作为该产品质量控制的方法。

AIM： To establish an HPLC method for determining geniposide and liquiritin in Zhizi Baipi Decoction （ Fructus Gardeniae, Cortex Phellodendri Chinensis, Radix et Rhizoma Glycyrrhizae ）. METHODS ： A Kromasil Cls Column （4.6 mm ×250 mm, 5 um） was used. The mobile phase was methanol-0.5% glacial acetic acid for gradient elution. The flow rate was 1.0 mL/min, ）λ1 = 238 nm （detect the geniposide）, λ2 = 276 nm （detect the liquiritin）. The column temperature was at 30 ℃. RESULTS： The linear range of geniposide was within 49.1 ug/mL - 147.4 ug/mL, r = 0. 999 8, the recovery was 99.40% （ n = 9）. The linear range of liquiritin was within 6.1 ug/mL - 18.4 ug/mL, r =0. 999 9, the recovery was 99.94% （n =9）. CONCLUSION： This method has good reproducibility. It can be used for quality control in the production of Zhizi Baipi Decoction.