高效液相法测定板蓝根药材中三种核苷的含量

Determination of three nucleosides in Radix isatidis by HPLC

目的:测定板蓝根药材中尿苷、鸟苷、腺苷的含量。方法:采用Prevail C18(4.6 mm×250 mm,5μm)色谱柱;流动相:A(水)-B(乙腈);梯度洗脱:0~34 min(2%B^20%B);柱温:25℃;流速为:0.5 mL/min;检测波长:254 nm。结果:尿苷、鸟苷、腺苷分别在1.92~38.4μg/mL,1.75~35μg/mL,2.12~42.4μg/mL范围内线性关系良好,相关系数均为0.999 9。药材提取方法的平均回收率分别为99.64%,98.98%,98.85%,精密度、稳定性及重现性均良好。结论:所建立的方法灵敏快速,操作简便,重现性好,可用于板蓝根药材的质量控制。

AIM： To establish a method for determining guanosine, ufidine, and andadenosine for Radix Isatid/s. METHODS： The Prevail C18 analytical column（4.6 mm× 250 mm,5 um）was used . The mobile phases con- sisted of water and acetonitrile with gradient. The flow rate was 0.5 mL/min and column temperature was at 25 ℃. The detection wavelength was at 254 nm. RESULTS： The method had good linear relationship within the range 1.92 - 38.4 ug/mL; 1.75 - 35ug/mL ; 2.12 - 42.4 ug/mL of guanosine, uridine and andadenosine, respectively, with the correlation coefficients of 0.999 9. The average recoveries of the three nucleosides were 99.64% （RSD =0.38% ）, 98.94% （RSD =0.32% ） and 98.85% （RSD =0.48% ）, correspondingly. CONCLUSION： The method is fast, rapid, convenient, accurate and can be used for quality control of Radix isatidis.