电针及氟西汀对抑郁大鼠行为学及海马神经元凋亡的影响

Effects of electroacupuncture and fluoxetine on behavior and apoptosis of hippocampal cells in chronic stress rat

目的探究电针及氟西汀对慢性应激抑郁大鼠行为学及海马神经元凋亡的影响。方法65只Sprague—Dawley雄性大鼠，按随机数字表随机分为空白组、空白电针组、模型组、电针组、氟西汀组，每组13只。慢性应激后进行行为学评价；采用膜联蛋白V-异硫氰酸荧光素／碘化丙啶双染法检测海马神经元凋亡率；采用免疫组织化学方法检测海马bcl-2蛋白表达。结果模型组大鼠旷场试验水平穿越格数[（1．6±1．3）格]、竖立次数[（0．4±0．2）次]、体质量增加量[（34±18）g]均明显低于空白组[分别为（51．1±22．3）格、（13．2±4．6）次、（128±21）g]，P均〈0．05；海马神经元细胞凋亡率[（67±10）％]高于空白组[（53±13）％]，P〈0．05；海马组织bcl-2蛋白阳性细胞计数[（28±10）个／mm^2]低于空白组[（78±22）个／mm^2]，P〈0．05。电针组[分别为（39．3±14．3）格，（9．6±4．1）次，（81±43）g]、氟西汀组[分别为（37．2±15．1）格，（9．3±4．6）次，（80±35）g]上述指标均明显高于模型组（P〈0．05）；海马神经元细胞凋亡率[电针组（30±9）％，氟西汀组（51±13）％]均低于模型组（P〈0．05）；海马组织bel-2蛋白阳性细胞计数[电针组（56±18）个／mm^2，氟西汀组（62±24）个／mm^2]均高于模型组（P〈0．05），而电针组与氟西汀组间的差异无统计学意义（P〉0．05）。结论电针和氟西汀可改善抑郁大鼠行为学症状，这种改善与海马细胞凋亡机制有一定相关性。

Objective To study the effects of electroacupuneture and fluoxetine on apoptosis in the hippocampus in chronic stress depression rats. Methods Sixty-five Sprague-Dawley male rats were randomly divided into control group, control group combining with electroacupuncture, model group, electroaeupuncture group and fluoxetine group. The model was produced by chronic unpredictable mild stress. Open-filed test was used to detect the behavior of rats. Annexin V-FITC/ PI method was used to detect the apoptotie rates in the hippoeampus. Immunohistoehemical technique was used to detect the expression of bcl-2 protein. Results After stress, the model group rats＇ crossing numbers （ 1.6 ± 1.3 ）, rearing times （0. 4 ±0. 2） , body weight changes（34 ± 18） g were markedly less than that of control group rats＇ [ （51.1 ±22. 3）times, （ 13.2 ±4. 6）times, （ 128 ± 21 ）g; P 〈0. 05], and the hippoeampal apoptotic rates and the expression of bel-2 was higher in model group than control group （ P 〈 0.05 ）. In comparison with the model group, the crossing times, rearing times, body weight changes increased in eleetroacupuncture group [ （39. 3 ± 14. 3 ） times, （ 9. 6 ± 4. 1 ） times, （ 81 ± 43 ） g ] and fluoxetine group （37.2±15.1）times, （9.3 ±4.6）times, （80±35）g], （P〈0.05）, and the apoptotie rates of the electroaeupuncture group and fluoxetine group were lower （ P 〈 0. 05 ）, the expression of bel-2 were higher （P 〈 0. 05）. Those indexes have not apparently differences between electroacupuncture group and fluoxetine group （ P 〉 0. 05）. Conclusion Electroacupuncture and fluoxetine treatment could improve the behavior of depression induced by stress, which may be related to hippocampal apoptosis.