小青龙汤对哮喘豚鼠肺及胸腺的神经生长因子表达作用研究

Effect of Xiaoqinglong decoction on expression of NGF in lung and thymus in asthmatic guinea pigs

目的探讨小青龙汤对哮喘豚鼠肺及胸腺的神经生长因子(NGF)表达的影响。方法采用免疫组织化学和RT-PCR法,观察正常对照组、哮喘组、小青龙汤组、氨茶碱组及小青龙汤组配氨茶碱组的豚鼠肺和胸腺NGF表达的变化。结果哮喘组豚鼠NGF在肺和胸腺表达明显高于正常组,差异具有统计学意义(P<0.05),而小青龙汤组则能明显降低豚鼠NGF在肺和胸腺表达。结论NGF可能参与哮喘的发病过程,小青龙汤抑制哮喘豚鼠NGF的表达可能是其治疗哮喘的作用机制之一。

Objective To explore the effect of Xaoqinglong decoction on the expression of NGF on the lung and thymus in asthmatic guinea pigs.Methods The change of NGF expression were observed with immunohistochemistry and RT-PCR in the 1ung and thymus of all guinea pigs.Results The expression of NGF in the lung and thymus of asthmatic guinea pigs in asthmatic group was much higher than that in control group and the expression of NGF in Xiaoqinglong group was much lower than that in asthmatic group.Conclusion The NGF is possible to participate in the pathogenesis of the bronchial asthma,and the treating mechanisms of Xiaoqinglong decoction for asthma may be through inhibiting the expression of NGF.