角化棘皮瘤和高分化鳞状细胞癌p53和Bcl-2表达的比较研究

Comparative study on expression of p53 and Bcl-2 in keratoacanthoma and well-differentiated squamous cell carcinoma

目的研究p53、Bcl-2蛋白在角化棘皮瘤和高分化鳞状细胞癌中的表达及意义。方法采用EnVision免疫组化二步法对12例角化棘皮瘤、8例高分化鳞状细胞癌,进行p53、Bcl-2的表达检测。结果p53蛋白在角化棘皮瘤和高分化鳞状细胞癌中的阳性表达率分别为50.0%和62.5%,两者比较无显著性差异(P>0.05);Bcl-2蛋白在角化棘皮瘤和高分化鳞状细胞癌中的阳性表达率分别为66.7%和87.5%,两者比较无显著性差异(P>0.05)。结论p53和Bcl-2蛋白均参与了角化棘皮瘤和高分化鳞状细胞癌的发病过程,但不宜单独作为角化棘皮瘤和高分化鳞状细胞癌的鉴别诊断标志。

Objective It is to investigate the expression and its significance of p53 and Bcl- 2 in keratoacanthoma （KA） and well-differentiated squamous cell carcinoma （SCC）. Methods The expression of p53 and Bcl - 2 in 12 cases with KA and 8 cases with SCC were detected by the method of EnVision immunohistochemical technique. Results The positive expression rates of p53 in KA and SCC were 50 % and 62.5 % respectively, there was no significant difference in them （ P 〉 0.05 ）. The positive expression rates of Bcl - 2 in KA and SCC were 66.7 % and 87.5 % respectively, there was no significant difference in them （P 〉 0.05）. Conclusion p53 and Bcl- 2 may be involved in the pathogenesis of KA and SCC, but can not be used as good markers for differential diagnosis between KA and SCC.