藏雪莲水提取物对小白鼠组织中一氧化氮含量的影响

Effects of Tibetan Saussurea involucrata Kar.et Kir.Water Extract on the Content of NO in Mice Tissues

[目的]研究藏雪莲水提取物对小白鼠组织中一氧化氮含量的影响,为藏雪莲抗缺氧机制的研究提供参考。[方法]将100只小白鼠随机分为高剂量组、中剂量组、低剂量组和对照组,连续24 d分别灌服不同剂量的藏雪莲水提取物和生理盐水,然后处死小白鼠快速采取肝、肺、心肌和骨骼肌,测定各组织内一氧化氮的含量。[结果]结果表明,高剂量组的肝、肺和骨骼肌和中剂量组的肝中一氧化氮含量中显著高于对照组和低剂量组,差异极显著(P<0.01);中剂量组的肺和骨骼肌中一氧化氮含量高于对照和低剂量组,差异显著(P<0.05);对照组的心肌中一氧化氮含量高于各药物组,差异极显著(P<0.01)。[结论]藏雪莲能够提高小白鼠肝、肺和骨骼肌中一氧化氮的含量,降低心肌中的含量。

[ Objective ] The research aimed to study the effects of Tibetan Saussurea involucrata Kar. et Kit. water extract on the content of NO in mice tissues and provide the references for its anti-hypoxia mechanism. [ Method] 100 mice were randomly divided into high-dose group, middle dose group, low-dose group and the control group. The content of NO in liver, lung, cardiac muscle and skeletal muscle was detected after oral administration of different doses Tibetan Saussurea involucrata Kar. et Kir. water extract for treatment groups and saline water for control group in 24 days respectively. [ Result] The results indicated that the content of NO in liver, lung and skeletal muscle of high -dose group and in liver of middle dose group were significantly higher than that of control group and low-dose group （ P 〈 0.01 ）. The content of NO in lung and skeletal muscle in middle dose group were higher than that of low-dose group and the control group（ P 〈 0.05 ）. The content of NO in cardiac muscle in control group was higher than that of other group significantly （P 〈 0.01 ）. [ Conclusion~ Tibetan Saussurea involucrata Kar. et Kit. water extract can increase the content NO in liver, lung and skeletal muscle, and debase the content of NO in cardiac muscle in mice.