Medtronic导管建立动脉损伤模型及对内膜增生的观察

Observation of Intimal Thickening in Rat Models of Carotid Artery Injury Established with Medtronic Balloon

目的:采用Medtronic球囊建立大鼠颈动脉球囊损伤模型并观察损伤血管内膜的增生情况。方法:取35只雄性SD大鼠行左颈总动脉球囊内膜剥脱术建立动脉球囊损伤模型,分别于术后即刻、3、7、14、21、28和56d各取5只大鼠颈动脉损伤段和对侧正常动脉行病理切片及HE染色,观察内膜增生,图像分析软件分析内膜中膜的面积比值(I/M)。结果:球囊损伤使大鼠颈总动脉内膜剥脱和新生内膜增生,管腔狭窄。损伤后7d内膜开始增生,内膜面积0.129±0.010mm(2P<0.01),14～28d增生最明显(P<0.01),28～56d增生程度无明显差异(P>0.05);损伤后I/M比值逐渐增大,28d时I/M比值是7d时的6.5倍(P<0.01),为14d的1.7倍(P<0.01),56d与28d比较I/M比值无统计学差异(P>0.05)。结论:采用Medtronic球囊建立大鼠颈动脉球囊损伤模型简便、有效,可动态观察血管成形术后再狭窄的变化。

Objective： To observe the changes of intimal thickness in rat models of carotid artery injury established with medtronic balloon. Methods： Thirty-five male SD rats received balloon endarterectomy of the left carotid arteries. Immediately or at 3, 7, 14, 21, 28 and 56 d after injury, every five rats were sacrificed and harvested for the left injured carotid artery and right normal artery which were used in HE-stained pathological study of the intimal thickness. The pathological pictures were analyzed using special software in terms of intima to media area ratio （I/M）. Results ： Irregular intimal thickening and stenosis of artery cavity were observed since 7 d after balloon endarterectomy with the intima area 0.129±0.010 mm^2（p〈0.01）, became pronounced between 14-28 d （P〈0.01） and seemed to remain stable between 28-56 d （P〉0.05）. Post-injury I/M ratio increased over time to 6.5-fold of day 7 and 1.7-fold of day 14 after 28 days （P〈0.01） but remained stable thereafter through day 56 （P〉0.05）. Conclusion： The rat carotid artery injury model established with Medtronic balloon procedure proved to be easy and effective for continuous study of restenosis after artery injury.