LVLX-SDS体系的荧光特性及左氧氟沙星的测定

The fluorescence characteristic of LVLX-SDS and the determination of levofloxacin

目的研究不同表面活性剂对左氧氟沙星(levofloxacin,LVLX)荧光强度的影响,建立表面活性剂增敏,荧光光度法测定左氧氟沙星含量的新方法。方法比较不同表面活性剂对左氧氟沙星荧光特性的影响,发现在pH6.0的缓冲溶液中十二烷基硫酸钠(SDS)对左氧氟沙星荧光强度有显著的增敏作用,建立SDS增敏,荧光光度法快速测定左氧氟沙星的新方法。结果将该方法用于左氧氟沙星片剂和注射液的测定,结果与国标检测方法高效液相色谱(HPLC)法一致,样品平均回收率为100.4%,RSD为0.64%,检出限为18.9μg/L。结论该方法简便、快捷、准确。

Objective Effect of different surfactants on the fluorimetric intensity of levofloxacin （LVLX） were studied. We established a new method for quantitative determination of levofloxacin. Methods Effect of different surfactants on the fluorimetric intensity of levofloxacin have been compared. We discovered sodium dodecyl sulfate could enhance the fluorimetric intensity of levofloxacin. Based on this reaction, levofloxacin was determined by fluorescence spectrophotometry. Results The reaction conditions were investigated in detail. In I.VLX-SDS system, the linear range was 5-4,500 μg/L. and regression equation was F= 1.34339＋0. 09311C （μg/L） （r=0. 9994）. The average recovery of levofloxacin was 100.4% with RSD of 0. 64%. Conclusion This method was simple, accurate, rapid and could be used to determine ciprofloxacin in tablet and injection.