诺必擂停的电化学性质研究及示差脉冲伏安法测定

Electrochemical behavior of nobiletin and its detection by differentical pulse voltammetry

研究了抗癌药物诺必擂停在玻碳电极上的电化学行为及其反应机理,建立了一种灵敏的测定诺必擂停的分析方法。实验结果表明,在pH 2.0的磷酸盐缓冲溶液中,诺必擂停在-0.905 V电位处产生灵敏的还原峰,该还原峰电流与诺必擂停的浓度在8.21×10^-6-1.09×10^-4mol/L范围内呈现良好的线性关系（r=0.9923）,检出限为5.47×10^-6mol/L。该法已用于人血清中诺必擂停的测定,回收率在94.5%-104.4%之间。

The electrochemical behavior of nobiletin at a glassy carbon electrode has been studied by cyclic voltammetry in pH 2.0 PBS solution. The experimental results indicated that the number of electron and proton involved in this electrochemical reation was 1. A novel method was proposed for the determination of nobiletin by differential putsed vohammetry （DPV）. The peak currents were well-proportional to the concentration of nobiletin over the range from 8.21 × 10^-6 to 1.09 × 10^-4 mol/L （r = 0.9923） with a detection limit of 5.47 × 10^-6 mol/L by differentical pulsed voltammetry. The method has been used for the detection of nobiletin in human blood serum samples and the results were satisfactory.