荷移分光光度法测定头孢拉定

Spectrophotometric determination of cefradine based on the charge transfer reaction

采用紫外可见分光光度法研究了头孢拉定与茜素之间的荷移反应,从而建立了一种快速简便测定头孢拉定的荷移分光光度法。实验表明,在乙醇-水介质中,头孢拉定与茜素在30℃水浴中反应45 min后形成稳定的1∶1型荷移络合物,该络合物的λmax=524 nm,表观摩尔吸光系数为2.01×10^4L.mol^-1.cm^-1。头孢拉定药物质量浓度在0.87-87μg/mL范围内服从比尔定律,r=0.9990。相对标准偏差为1.1%（n=7）,加标回收率为91.4%-103.6%。

This paper established a quick, simple and convenient method to determine cefradine based on charge transfer spectrophotometry through the study on the charge transfer reaction between cefradine and alizarin by UV-Vis spectrophotometry. After being placed in the 30 ℃ water bath for 75 min, cefradine can react with alizarin to form 1 ： 1 complex in the presence of acohol-water solution, which gives a maximum absorption peak at 524 urn. The molar absorptivity of the complex is 2.01 × 10^4 L.mol^- 1 . cm^- 1 and the linear range is 0.87 - 87 μg/mL. RSD is 1.1% （ n = 7）. The recoveries are between 91.4% and 103.6%.