基于离子液体-石蜡油混合物为黏和剂的酪氨酸酶碳糊生物传感器的研制

Construction of a novel tyrosinase carbon paste biosensor using mixture of ionic liquid and paraffion oil as the binder

以离子液体（1-丁基-3-甲基咪唑六氟磷酸盐、[BMIM]PF6）和石蜡油的混合物为碳糊电极（CPE）黏和剂,固定酪氨酸酶（Tyrosinase）,制备了一种新型酪氨酸酶碳糊生物传感器（T-CPE-IL）。T-CPE-IL较传统酪氨酸酶碳糊电极（TCPE）对邻苯二酚的响应灵敏度有明显提高,且电极响应快、稳定性好。T-CPE-IL峰电流的增加与邻苯二酚浓度在20-200μmol内呈良好的线性关系,检出限可达3μmol。同时,FT-IR的初步研究表明,[BMIM]PF6对酪氨酸酶的二级结构产生了一定的影响。

A novel tyrosinase carbon paste biosensor （T-CPE-IL） was constructed with paraffin oil and ionic liquid, 1- butyl-3-methyl-imidazolium hexafluorophosphate （BMIMPF6）, as binder for carbon paste. The proposed biosensor presented higher sensitivity and faster response for catechol than that of traditional carbon paste biosensor. Under the optimum conditions, the increase of the peak current ip of T-CPE-IL was linear with the concentrations of catechol in the range of 20 - 200μmol, and the detection limits was 3μmol. FT-IR spectra showed that the secondary structure of the enzyme changed when tyrosinase was mixed with [BMIM]PF6.