电化学法研究橙黄Ⅳ和溶菌酶的相互作用

Study on Interaction of orange yellow Ⅳ and bacteriolysin by linear sweep second derivative polarography

在pH 11.00的NH3-NH4Cl缓冲溶液中,橙黄Ⅳ与溶菌酶能形成一种橙黄色的超分子复合物。用线性扫描二阶导数极谱法对该体系进行了研究,复合物的形成使橙黄Ⅳ的还原峰电位不变,峰电流下降,并且该峰电流的下降值同所加的溶菌酶的浓度在一定范围内呈线性关系。用于溶菌酶的测定,在1.6×10^-9-1.0×10^-8mol/L,1.0×10^-8-8.0×10^-8mol/L,9.0×10^-8-1.6×10^-7mol/L三段范围内呈线性关系,检出限为4.0×10^-10mol/L,进而对模拟样品进行分析,并对结合反应机理进行初步的探讨。

Orange yellow Ⅳ is an azo reagent, with very strong electrochemical activity and its can be used as an electrochemical probe. In this work, in the pH 11.00 NH3-NH4Cl buffer solution, the orange yellow Ⅳ can form one kind of orange yellow supermolecular compound with bacteriolysin. The system was studied by linear sweep second derivative polarography, and it was found that the formation of the complex did not change the peak potential of orange yellow Ⅳ, and the peak current decreased, furthermore, the decrease value of the peak current has a linear relationship with the concentration of bacteriolysin added. The linear ranges are 1.6 × 10^-9 - 1.0 × 10^-8 mol/L, 1.0 × 10^-8 × 8.0 × 10^-8 mol/L and 9.0 × 10^-8 - 1.6 × 10^-7 mol/L, respectively. The detection limit is 4.0 × 10^-10 mol/L. The proposed method has been used to analyze the samples of bacteriolysin. The possible mechanism of the interaction between orange yellow Ⅳ and bacteriolysin was discussed.