海洋无脊椎动物中α-葡萄糖苷酶抑制剂的初步研究

Screening for α-glucosidase inhibitors from marine invertebrates extracts

目的对青岛地区的海洋无脊椎动物粗提物进行α-葡萄糖苷酶抑制剂的初步筛选。方法采用α-葡萄糖苷酶抑制剂活性筛选模型,对采自青岛沿海的33种海洋无脊椎动物乙醇粗提物进行了活性筛选。结果33种海洋无脊椎动物粗提物中,有10种具有不同程度的α-葡萄糖苷酶抑制剂活性,其中海筒螅、海燕、刺参和毛蚶的粗提物表现出较强的活性,在浓度为0.6 mg.mL-1时,分别为56.1%、67.3%、62.2%、66.3%。结论某些海洋无脊椎动物中存在具有α-葡萄糖苷酶抑制活性的成分,值得进一步研究开发。

Objective To screen for α-glucosidase inhibitors from the extracts of marine inverte- brates collected at the seashore of Qingdao China. Method The 95% ethanol extracts of 33 species of marine invertebrates were screened for the presence of α-glueosidase inhibitors using rapid colorimetric method. Result Some extracts have inhibitory activity against α-glucosidase, among which the extracts of Tubularia marina, Asteri＇na pectinifera, Apostichopus japonicus and Scapharca subcrenata have much higher inhibitory rate. 56. 1%,67. 3%, 62.2%, and 66.3% at the concentration of 0.6 mg.mL^-1. Conclusion The results suggested that marine invertebrates are sources of new α-glucosidase inhibitors.