细棒束孢培养物对小鼠移植性肿瘤抑制作用的实验研究

Experimental Study on the Inhibitory Action of Isaria Feline Culture on the Transplantation Tumor of Mice

目的:观察细棒束孢Isaria feline(DC.:Fr.)Fr.培养物对小鼠移植性肉瘤S180、肝癌H22、Lewis肺癌的抑瘤作用,探讨其应用于肿瘤临床的可行性。方法:采用肿瘤移植法建立小鼠荷瘤动物模型,灌胃给药,剂量为3.0g/kg、1.5g/kg、0.75g/kg,连续给药10d,观察药物对肿瘤生长的抑制作用,以环磷酰胺作为阳性对照药。结果:0.75g/kg、1.5g/kg、3.0g/kg剂量组对S180的抑瘤率分别为2.43%、34.84%(P<0.01)和47.03%(P<0.01);对H22的抑瘤率分别为12.24%、28.06%和36.22%(P<0.01);对Lewis肺癌的抑瘤率分别为24.57%、36.44%(P<0.01)和43.22%(P<0.01)。结论:细棒束孢培养物对小鼠移植性肉瘤S180、肝癌H22和Lewis肺癌均有显著的抑瘤作用,是一种极具开发前景的抗肿瘤新药资源。

Objective：To observe the inhibitory action of Isaria feline（DC.： Fr.）Fr.culture on the transplantation sarcoma S180,liver cancer H22,and Lewis lung cancer of mice and probe the feasibility of its clinical application.Methods：The mice models were made by tumor transplantation.The medicine was given by stomach feeding with the doses of 3.0 g/kg,1.5 g/kg and 0.75 g/kg in succession for 10 days in order to observe its inhibitory action on tumor growth.The endoxan was used in the control group.Results：The inhibitory rate of 0.75 g/kg,1.5 g/kg and 3.0 g/kg dosage groups on S180 was 2.43%,34.84% and 47.03% respectively（P〈0.01）; on H22 was 12.24%,28.06% and 36.22% respectively（P〈0.01）; and on Lewis lung cancer was 24.57%,36.44% and 43.22% respectively（P〈0.01）.Conclusion：Isaria feline （DC.： Fr.）Fr.Culture has an obvious inhibitory action on the transplantation sarcoma S180,liver cancer H22,and Lewis lung cancer of mice.It is a new medicine of anti-tumor with a splendid prospect in clinical application.