期刊文献+

灰绿黄堇总生物碱对实验性溃疡性结肠炎的作用研究 被引量:4

The effect of alkaloids of corydalis adunca maxim on experiment ulcerative colitis induced by dextran sulfate sodium
下载PDF
导出
摘要 目的研究灰绿黄堇总生物碱(ACAM)对小鼠结肠炎的作用及其机制。方法实验设正常、模型、柳氮磺胺吡啶组(SASP,520mg/kg)和ACAM组(100、200、400mg/kg)。正常组小鼠饮用蒸馏水,其余组自由饮用4%葡聚糖硫酸钠(DSS)水溶液,同时分别灌胃给予溶剂或干预药物(0.2ml/10g,1次/d×7d)。记录小鼠疾病活动指数(DAI);测定结肠组织MDA含量,SOD、MPO活性及ICAM-1、NF-κBp65表达水平。结果模型组DAI显著增高,结肠黏膜损伤严重;MDA舍量、MPO活性及ICAM-1和NF-κBp65表达明显升高。SOD活性下降(P<0.01)。SASP520mg/kg能明显逆转上述改变;ACAM100mg具有相似的作用。结论ACAM可能通过抗氧自由基作用,抑制炎性细胞活化、迁移及NF-κB激活,缓解小鼠结肠炎性反应。 Objective To investigate the effect of alkaloids of corydalis adunca maxim(ACAM) on experimental ulcerative colitis in mice. Methods BALB/C mice of 6 groups were allowed to drink either 4% dex- tran sulfate sodium (DSS) solution or distilled water freely with different doses of ACAM (50,100,200 mg/kg) or salicylazosulfapyridine( SASP,520 mg/kg), and solvent(0. 2 ml/10 g)once a day for 7 days, respectively. The symptom of ulcerative colitis was evaluated by disease activity index (DAI), Myeloperoxidase (MPO)and superoxide dismutase(SOD) activities and malondialdehyde(MDA) content were determined by HE staining and im- munohistochemistry of expressions of NF-KB p65 and intercellular adhesion molecule 1 ( ICAM-1 ) proteins to ob- serve the damage of colon tissues and. possible mechanisms. Results DAI, MPO activity, MDA content and expressions of ICAM-1 and NF-KB were markedly increased, while SOD activity decreased in DSS-treated mice. Treatment of different doses of ACAM or SASP in mice decreased significantly DAI, MPO activity and MDA con- tent,improved histological changes of colon tissues, blunted the expressions of NF-KB p65 and ICAM-1 proteins, and promoted SOD activity. Conclusion ACAM has excellent therapeutic effect on ulcerative colitis caused by DSS. The possible mechanism may be related to its antioxidant and anti-inflammatory activities associated with inhibiting the NF-KB activation and ICAM-1 expression.
作者 马国中
出处 《中国实用医药》 2008年第29期8-10,共3页 China Practical Medicine
关键词 葡聚糖硫酸钠 灰绿黄堇总生物碱 结肠炎 Dextran sulfate sodium Alkaloids of corydalis adunca maxim Colitis
  • 相关文献

参考文献9

  • 1张莉,刘玮.灰绿黄堇的鉴别[J].基层中药杂志,2002,16(4):39-40. 被引量:5
  • 2[2]杨永昌.藏药志.西宁:青海入民出版社,1991:358.
  • 3王继生,张莉,邱宗荫,夏永鹏.灰绿黄堇镇痛抗炎作用研究[J].中药药理与临床,2005,21(5):33-34. 被引量:7
  • 4王继生,张莉,邱宗荫,等.灰绿黄堇总生物碱镇痛抗炎作用的实验研究[J].中国药房,2007,19(7):468-470.
  • 5[5]Krawisz JE,Sharon P,Stenson WF,et al.Quantitative assay for acute intestinal inflammation based on myeloperoxidase activity.Assessment of inflmmaiton in tat and hamster mod-els.Gastroenterology,1984,87(6):1344.
  • 6[6]Neurath NF.Pathogenesis of inflammotory bowel disease:tran-scripinon factors in the spotilight.Guit,1998,42(4):458.
  • 7[7]Wong PY,Yue G,Yin K,et al.Antibodies to intercellular adhesion molecule-l ameliorate the inflammatory response in acetic acid-induced inflammatory bowel disease.J Phar-macol Exp Ther,1995,274(1):475.
  • 8[8]Minami T,Abid MR,Zhang J,et al.Thrombin stimulation of vascular adhgesion molecule-l in endothelial cells is mediater by protein kinase C(PKC)-delta-NF-kappa B and PKC-zeta-GATA signaling pathways.J Biopl Chem,2003,278(9):69-76.
  • 9[9]Tamanini A,Rolfini R,Nicolis E,et al.MAP kinases and Nf-kappaB collaborate to induce ICAM-l geneexpressinon in the early phase of adenovirus infection.Virology,2003,307(2):228.

二级参考文献3

共引文献10

同被引文献115

引证文献4

二级引证文献49

相关作者

内容加载中请稍等...

相关机构

内容加载中请稍等...

相关主题

内容加载中请稍等...

浏览历史

内容加载中请稍等...
;
使用帮助 返回顶部