秸秆水解物乙醇发酵菌群功能强化

Construction and function testing of an new ethanol fermentative microbial system using corn stalk hydrolysate

在人工筛选得到的4株野生乙醇发酵酵母菌株基础上,加入康宁木霉,运用正交实验方法研究了菌群对乙醇发酵的影响。结果表明转速、转速×温度交互作用对菌群生物量形成具有极显著影响;Saccharomyces kluyveri菌及其与Cryptococcus flavescens菌之间的相互作用对生物量形成具有显著影响;Saccharomyces kluyveri菌株对乙醇形成具有显著影响,是乙醇发酵过程中的关键菌,其他因子对乙醇生成量影响不大;菌群直接发酵秸秆水解物,能获得12.19 g/L的乙醇。

The influences of mixed microbes during the ethanol fermentation process by the orthogonal experiment are reported, in which four kinds of yeast strains collected from orchard soil and one cellulose hydrolyzing microbe, Trichoderma konigii are used as raw materials. The results show that： （1）rotate speed, interaction between rotate speed and temperature have notable influence on biomass. The selected microbial strain Saccharomyces kluyveri and its interaction with Cryptococcus flavescens have prominent influence on biomass; （2） S. kluyveri stain has remarkable positive influence on ethanol yields. The other factors have no remarkable influence on ethanol yields. S. kluyveri strain is the key microbial strain during the ethanol fermentation process. （3） 12.19 g/L of ethanol can be acquired during the fermentation of corn stalk hydrolysates by combinatorial microbes.