全酶法制备甘油二酯工艺放大研究

Scale-up study on production process of diacylglycerol by holoenzymatic process

通过对酶催化部分水解反应和酶催化酯化反应的有效整合,提出发展了全酶法制备甘油二酯的专利工艺。在前期实验室研究的基础上,进行全酶法制备甘油二酯整套工艺的放大研究。多批次的小试、中试实验结果与实验室研究的结果比较相近,采用游离酶催化油脂部分水解反应及采用填充床酶反应器的酯化反应还具有反应效率较高、工艺操作简单、反应重现性好、生产成本低廉等优点,是制备中、高含量甘油二酯产品的适宜方法;反应后的半成品经过分子蒸馏的分离、提纯及进一步的精制,可以获得符合相关国家标准的甘油二酯产品。

The Patented holoenzymatic process which combines the enzymatic controlled hydrolysis with enzymatic esterification is developed to produce diacylglycerol. The successive scale-up of diacylglycerol production using the holoenzymatic process from laboratory-scale （gram） to pilot-scale （kilogram） is carried out,based on the early research on this process. Similar results of laboratory studies are achieved when the process is sealed up, indicating that scale-up of the whole process is successful, and the liquid lipase-eatalyzed partial hydrolysis and immobilized lipase-catalyzed esterification has the advantages of higher reaction efficiency, simpler process operation, excellent recurrence of reaction, lower operating costs, etc. The holoenzymatic process is a feasible method to synthesize a series of diacylglyeerol product. Diacylglycerol product in accord with the relatively national standard is obtained after molecular distillation, purified and semi-finished product further refined.