bFGF对大鼠成骨细胞膜integrin α_2、α_5、β_1 mRNA表达的调控作用

Regulation of Integrin α_2,α_5,β_1 mRNA Expression on Osteoblasts Membrane by bFGF

目的探讨外源性碱性成纤维细胞生长因子(bFGF)对体外培养大鼠成骨细胞膜上整合素(integrin)亚基mRNA表达的影响。方法用含不同浓度bFGF F12培养基对大鼠成骨细胞进行24 h预孵后,接种于喷砂处理的钛片上,第3 d收集成骨细胞,利用实时荧光定量RT-PCR的方法检测成骨细胞膜上integrin α2、α5、β1 mRNA的表达。结果体外培养的大鼠成骨细胞膜上integrin β1 mRNA表达水平最高,其次为integrin α2,integrin α5相对最低。bFGF能不同程度有效上调成骨细胞integrin α2、α5、β1 mRNA的表达,但促进integrin α2、α5、β1 mRNA表达的bFGF的有效浓度范围不同。结论bFGF能促进大鼠成骨细胞膜上integrin α2、α5、β1 mRNA的表达。

Objective To observe the regulation of bFGF on mRNA expression of integrin subunits α2 ,α5,β1 in osteoblasts. Methods Osteoblasts were pre-cultured for 24 h with F12 medium containing hFGF with different concentration and planted on the sand blasting titanium disks. Three days after planting, osteoblasts were harvested. The mRNA expression of integrin suhunit α2 ,α5,β1 were examined by immunofluorescent quantitative real-time polymerase chain reaction （RT-PCR）. Results The mRNA expression of integrin β1 is most abundant and the mRNA expressions of integrin α2, α5 are relatively lower than integrin β1. In the study, the mRNA expression levels of integrin α2, α5 and β1 were up-regulated after bFGF stimulation. However, the effective concentration range of bFGF to the three subunits was different. Conclusion bFGF could up-regulate the mRNA expression of integrin subunits in osteoblasts.