氯苯丁酯对雌性肥胖Zucker大鼠心肌甘油三酯积聚的影响

Effect of Clofibrate on Myocardial Triglyceride Accumulation in Female Obese Zucker Rats

目的观察氯苯丁酯对雌性肥胖Zucker大鼠心肌甘油三酯积聚的影响,以探讨其在脂毒性心肌病中的应用。方法选择瘦型和肥胖型Zucker大鼠24只,分为:瘦大鼠+赋形剂组,瘦大鼠+氯苯丁酯组,肥胖大鼠+赋形剂组和肥胖大鼠+氯苯丁酯组,分别给予腹腔内注射氯苯丁酯(300 mg/kg)或赋形剂(橄榄油,1 mL/kg)2周。测定血糖和胰岛素浓度,血浆游离脂肪酸、甘油三酯、胆固醇浓度,以及心脏甘油三酯和脂肪酸含量。结果氯苯丁酯可使肥胖大鼠血浆游离脂肪酸和胆固醇浓度恢复正常并可降低血浆甘油三酯和胰岛素浓度,可增加肥胖大鼠心肌甘油三酯的积聚,且心肌长链不饱和脂肪酸和单不饱和脂肪酸含量也相应增加。结论氯苯丁酯可增加肥胖Zucker大鼠心肌甘油三酯积聚,使用其治疗脂毒性心肌病应严格控制。

Objective To determine the effect of clofibrate on myocardial triglyceride accumulation in female obese Zucker rats and its role in lipotoxic heart disease. Methods Twenty four female Zucker rats were divided into four groups., lean rats＋vehicle, lean rats＋clofibrate, obese rats ＋ vehicle, obese rats＋clofibrate. Daily intraperitoneal injection of clofibrate （300 mg/kg） or vehicle （olive oil, 1 mL/kg） was given to the rats for two weeks. Plasma glucose, insulin, free fatty acids, triglycerdles, cholesterol, and cardiac triglycerdies and fatty acids were measured. Results Clofibrate normalized plasma free fatty acid and cholesterol and reduced plasma triglyceride and insulin in obese rats. However, clofibrate led to accumulation of heart TG and increased myocardial long-chain unsaturated and monounsaturated fatty acids in obese rats. Conclusion Clofibrate aggravates myocardial triglyceride accumulation in female obese Zuckcr rats. Further studies are needed for the use of clofibrate in treating cardiac lipid accumulation.