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受体相互作用蛋白140敲低对小鼠小胶质瘤细胞增殖的影响 被引量：3

Effects of receptor-interacting protein 140 gene knockdown on the proliferation of microglioma cells： experiment with rat microglioma cells
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摘要 目的探讨受体相互作用蛋白（RIP）140基因敲低对小鼠小胶质瘤细胞（BV-2）增殖的影响。方法用脂质体转染方法将RIP140-短发夹RNA（shRNA）质粒导入BV-2细胞，用嘌呤霉素筛选稳定表达RIP140-shRNA的细胞系，用实时定量PCR和Western印迹法检测RIP140敲低的效果，用四甲基偶氮唑蓝（MTY）法检测RIP140敲低对细胞增殖的影响。结果成功构建稳定表达RIP140-shRNA的BV-2-71674和BV-2-71080；与BV-2细胞相比，BV-2-71674和BV-2-71080细胞中RIP140的表达无论mRNA水平还是蛋白质水平均明显低，下调率（mRNA）分别为73％和75％（均P〈0．01）；MTT法检测显示，BV-2细胞在24、48、72、96h 4个时间点增殖率分别为3．03±0．01、7．36±0．08、14．15±0．25、23．35±0．09，BV-2-71674细胞则分别为2．15±0．03、6．43±0．08、11．77±0．31、18．47±0．04，BV-2-71080细胞分别为1．77±0．03、4．74±0．25、10．03±0．02、17．66±0．21；BV-2-71674和BV-2-71080细胞增殖率均明显低于BV-2细胞，差异均有统计学意义（均P〈0．01）。结论RIP140敲低可以抑制BV-2细胞的增殖。 Objective To investigate the effects of receptor-interacting protein （RIP） 140 gene knockdown on the proliferation of microglioma cells. Methods Mouse microglioma cells of the line BV-2 were cultured and transfected with 2 kinds of recombinant RIP140-shRNA plasmids （V2MM-71674 and V2MM-71080） or blank plasmid MSCV-EGFP. Real-time PCR and Western blotting were used to detect the mRNA and protein expression of RIP140; and the cell proliferation was detected by MTT assay. Results There were not significant differences in the RIP140 mRNA and protein expression between the BV-2 and BV-2-MGCV-EGFP groups. Compared to those of the BV-2 group, the RIP140 mRNA expression levels of the BV-2-71674 and BV-2-71080 groups were lower by 73% and 75% respectively. The protein expression levels of the BV-2-71674 and BV-2-71080 groups were remarkably lower than those of the BV-2 and BV- MSGV-EGFP groups. MTT assay showed that there were not significant differences in the proliferation rates at different time points between the BV-2 and BV-2-MSCV-EGFP groups, however, the proliferation rates at the time points of 24, 48, 72, and 96 h of the BV-2-71674 and BV-2-71080 groups were significantly lower than those of the BV-2 group （ all P 〈 0. 01 ）. Conclusion RIP140 gene knockdown effectively inhibits the proliferation of microglioma cells.
作者 晁爽 郁卫东 杜军保 曾超美 梁蓉 杨丽君 陈敏霞 赵贺华 郭静竹
机构地区 北京大学人民医院儿科 北京大学人民医院临床分子生物学研究所 北京大学第一医院儿科
出处 《中华医学杂志》 CAS CSCD 北大核心 2008年第40期2857-2861,共5页 National Medical Journal of China
基金 基金项目：国家重点基础研究发展计划基金资助项目（2001CB510303）
关键词 神经胶质瘤 细胞增殖 受体相互作用蛋白140 Glioma Cell proliferation RIP140
分类号 R686 [医药卫生—骨科学]
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参考文献12

  • 1Bahn S, Mimmack M, Ryan M, et al. Neuronal target genes of the neuron-restrictive silencer factor in neurospheres derived from fetuses with Down's syndrome: a gene expression study. Lancet, 2002, 359:310-315.
  • 2Treuter E, Albrektsen T, Johansson L, et al. A regulatory role for RIP140 in nuclear receptor activation. Mol Endocrinol, 1998, 12: 864-881.
  • 3郁卫东,梁蓉,杨丽君,尚美,沈浣,关菁,郭静竹.致唐氏综合征胎儿大脑皮质发育异常的HC21基因的筛查和鉴定[J].中华医学杂志,2007,87(39):2759-2763. 被引量：6
  • 4Mrak RE, Griffin WS. Glia and their cytokines in progression of neurodegeneration. Neurobiol Aging, 2005, 26: 349-354.
  • 5Livak KJ,Schmittgen TD. Analysis of relative gene expression data using real-time quantitative PCR and the 2^-△△Ct method. Methods, 2001,25:402-208.
  • 6Klinge CM, Carolyn M. Estrogen receptor interaction with coactivators and co-repressors. Steroids, 2000, 65 : 227-251.
  • 7Chan S, Kilby MD. Thyroid hormone and central nervous system development. J Endocrinol, 2000, 165:1-8.
  • 8Kim SU, de Vellis J. Microglia in health and disease. J Neurosci Res, 2005, 81: 302-313.
  • 9Mann DM, Esiri MM. The pattern of acquisition of plaques and tangles in the brains of patients under 50 years of age with Down's syndrome. J Neurol Sci, 1989, 89: 169-179.
  • 10Mann DM, Iwatsubo T, Fukumoto H, et al. Microglial cells and amyloid beta protein (A beta) deposition; association with A beta 40-containing plaques. Acta Neuropathol, 1995, 90 : 472-477.

二级参考文献12

  • 1Gardiner K, Fortna A, Bechtel L, et al. Mouse models of Down syndrome: how useful can they be? Comparison of the gene content of human chromosome 21 with orthologous mouse genomic regions. Gene, 2003, 318:137-147.
  • 2Reeves RH. Functional genomics: a time and place for every gene. Nature, 2002, 420:518-519.
  • 3Antonarakis SE, Lyle R, Chrast R, et al. Differential gene expression studies to explore the molecular pathophysiology of Down syndrome. Brain Res Brain Res Rev, 2001, 36: 265-274.
  • 4Capone GT. Down syndrome: advances in molecular biology and the neurosciences. J Dev Behav Pediatr, 2001,22:40-59.
  • 5Gardiner K,Davisson M. The sequence of human chromosome 21 and implications for research into Down syndrome. Genome Biol, 2000,1: 1-9.
  • 6Gardiner K. Transcriptional dysregulation in Down syndrome: predictions for altered protein complex stoichiometries and posttranslational modifications, and consequences for learning/ behavior genes ELK, CREB, and the estrogen and glucocorticoid receptors. Behav Genet,2006 ,36 :439-453.
  • 7Cavailles V, Dauvois S, L'Horset F, et al. Nuclear factor RIP140 modulates transcriptional activation by the estrogen receptor. EMBO J,1995, 14:3741-3751.
  • 8Klinge CM, Carolyn M. Estrogen receptor interaction with coactivators and co-repressors. Steroids,2000, 65:505-512.
  • 9Chan S, Kilby MD. Thyroid hormone and central nervous system development. J Endocrino1,2000,165 : 1-8.
  • 10Morgane PJ, Austin-LaFrance R, Bronzino J, et al. Prenatal malnutrition and development of the brain. Neurosci Biobehav Rev, 1993,17 : 91-128.

共引文献5

同被引文献34

  • 1Treuter E,Albrektsen T,Johansson L,et al.A regulatory role for BIPl40 in nuclear receptor activation[J].Mol Endoefinol,1998,12:864-881.
  • 2Mrak RE,Griffin WS.Glia and their eytokines in progression of neurodegeneration[J].Neurobiol Aging,2005,26:349-354.
  • 3McCarthy KD,Devellis J.Preparation of separate astroglial and oligodendroglial cell cultures from rat cerebral tissue[J].J Cell Biol,1980,85:890-902.
  • 4Hao C,Richardson A,Fedoroff S.Macrophage-like cells origi nate from neuroepithelium in culture:characterization and properties of the macrophage like cells[J].Int J Dev Neu resei,1991,9:1-14.
  • 5Klillge CM,Carolyn M.Estrogen receptor interaction with co activators and co-repressors[J].Steroids,2000,65:227-251.
  • 6Wierzba-Bobrowicz T, Lewandowska E, Schmidt-Sidor B, et al. The comparison of microglia maturation in CNS of normal haman fetuses and fetuses with Down's syndrome. Folia Neuropathol, 1999,37:227-234.
  • 7Mao R, Zielke CL, Zielke HR, et al. Global up-regulation of chromosome 21 gene expression in the developing Down syndrome brain. Genomics,2003 ,81:457467.
  • 8Glasson EJ, Sullivan SG, Hussain R, et al. The changing surwival profile of people with Down' s syndrome : implications for genetic counselling. Clin Genet ,2002 ,62 : 390-393.
  • 9Bittles AH, Glasson EJ. Clinical, social, and ethical implications of changing life expectancy in Down syndrome. Dev Med Child Neurol, 2004,46 : 282 -286.
  • 10Cuadros MA, Navascues J. The origin and differentiation of microglial cells during development. Prog Neurobiol, 1998,56: 173-189.

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中华医学杂志
2008年 第40期

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