子宫内膜样腺癌间质细胞和癌细胞TGFβR/Smads的表达

Expression of TGFβR/Smads in endometrioid adenocarcinoma cancer cells and stromal cells

目的探讨子宫内膜间质细胞转化生长因子β受体(TGFβR)、Smad蛋白(Sma-and Mad-related protein)信号通路的状态和癌细胞TGFβR/Smads信号通路的关系,及其这一信号通路可能对子宫内膜癌发生发展的影响。方法采用SP免疫组化方法检测12例子宫肌瘤、18例子宫内膜样腺癌中TGFβRⅠ、TGFβRⅡ、Smad2、Smad3、Smad4、Smad7的表达。结果在正常子宫内膜细胞中均有TGFβRⅠ、TGFβRⅡ、Smad2、Smad3、Smad4、Smad7的表达,正常子宫内膜腺体上皮细胞和间质细胞中TGFβR蛋白与Smads蛋白的表达一致,两者成正相关(r=0.86,r=0.78);子宫内膜癌癌细胞和间质细胞中TGFβR/Smads蛋白的表达不一,子宫内膜癌本身的间质细胞TGFβR/Smads蛋白表达缺陷。结论正常子宫增生期/分泌期内膜细胞存在TGFβR/Smads信号表达,子宫内膜癌本身的间质细胞存在TGFβR/Smads信号缺陷;探讨内膜间质细胞TGFβR/Smads信号通路作用,为内膜癌的基因治疗提供了新的靶点。

Objective To investigate the relationship between the status of the TGFβR/Smads signal pathway in cancer cells and in its stromal cells, and if this may affect the development of endometrioid adenocarcinoma. Methods TGFβR Ⅰ , TGFβR Ⅱ , Smad2, Smad3, Smad4 and Smad7 were determined in hysteremyoma （12 cases） and endometrioid adenocarcinoma （18 cases） by the SP immunohistochemical method. Results TGFβR Ⅰ, TGFβR Ⅱ, Smad2, S mad3, Smad4 and Smad7 were found in hystemmyoma. TGFβR expression which was in positive correlation with Smads expression in normal endometrium cells（ r = 0.86, r = 0.78）. TGFβR/Smads expression disagreed in endometrial cancer cells and stromal cells and it was not found in stromal cells of endometrial adenocarcinoma per se. Conclusions TGFβR/Smads signals exist in cells of normal endometrial proliferative/secretory phase but its pathway is defected in tumor stroma. To get the information about TGFβR/Smads signal way in stroma proba bly provides a neotarget for gene therapy of endometrioid carcinoma.