滇产毛喉鞘蕊花提取成分对缓激肽诱导豚鼠肺微血管渗漏的影响

Effect of the Ingredients From Yunnan Native Plant Coleus Forskohlii on Bradykinin-induced Lung Microvascular Leakage in Guinea Pig

目的建立豚鼠肺微血管渗漏损伤模型,从动物水平上研究从滇产毛喉鞘蕊花提取的有效部位(CF-E,异佛司可林类似物的混合物)对微血管渗漏的影响,对其抗急性肺损伤(AL)I作用机理进行初步探讨.方法静脉注射缓激肽诱导豚鼠肺微血管渗漏,然后静脉注射伊文思蓝(EB),通过测定肺组织中渗漏的EB含量,反映微血管渗漏程度.灌胃给予豚鼠CF-E,观察受试验品对微血管渗漏的影响.结果缓激肽模型组的肺组织EB含量明显增加,证明缓激肽造成了豚鼠肺微血管渗漏反应;而CF-E低、中、高剂量组(50、100、200mg/k)g肺组织中EB含量显著低于模型组(P<0.01、P<0.05),显示CF-E对缓激肽造成的豚鼠微血管渗漏具有保护作用.结论CF-E可明显降低缓激肽诱导的豚鼠肺微血管渗漏反应,提示其通过抑制肺微血管渗漏,抑制肺水肿,从而具有ALI作用.

Objective In guinea pig lung microvascular leakage damage model, effect of active ingredients （CF-E） isolated from Yunnan native plant Coleus Forskohlii on lung microvascular leakage induced by bradykinin, was investigated to reveal it＇s mechanism of anti-acute lung injury （ALI）. Method Intravenously inject bradykinin to induce pulmonary microvascular leakage inject Evans blue （EB） , through testing of EB leaking level response in guinea pigs, and then intravenously in the lung tissue, microvascular leakage level and effect of CF-E was determined after CF-E intragastric administration. Results In Bradykinin-induced microvascular leakage model group, EB content in lung tissue was significantly increased, but was significantly decreased by CF-E （50, 100, 200 mg/kg） compared with control （P 〈 0.01, P 〈 0.05）. Conclusion CF-E significantly reduced bradykinin-induced lung microvascular leakage response in guinea pig, and this might be related to it＇ s prevention of lung edema and causing anti-related acute lung injury action.