秋水仙碱对胶质瘤细胞抑制作用的体外实验研究

Effects of Colchicine on Proliferation and Apoptosis of Rat Glioma G422 Cells in Vitro

目的研究秋水仙碱对G422胶质瘤细胞生长增殖的抑制作用.方法传代培养G422胶质瘤细胞,将其分为对照组、秋水仙碱处理组同时进行实验,秋水仙碱处理组的药物浓度分别为2、4、8、16μg/mL.分别在1、2、3d用MTT法测定肿瘤生存率.结果秋水仙碱能明显抑制G422胶质瘤细胞的生长,而且随着秋水仙碱的浓度增大及时间延长,肿瘤细胞的生存率也降低.秋水仙碱的浓度在64μg/mL,作用3d时,肿瘤细胞的生存率仅(16.97±1.32)%.结论秋水仙碱对G422胶质瘤细胞的生长增值有明显的抑制作用,并表现为浓度和时间依赖性.

Objective To study the effects of Colchicine on proliferation and apoptosis of rat glioma G422 cells in vitro. Methods The rat G422 cells were cultured in the RPMI 1640 medium, then 100 μL was added into 96 holes board by 3 holes per group, the cells were divided into many treatment groups （2, 4, 8, 16 μg/mL Colehicine and so on ） and blank control group, and MTT assay was used to observe the proliferation of the glioma C-422 cells treated for 24, 48 and 72 h each other. Results The proliferation of G422 glioma cells was suppressed in a concentration and time-dependent manner, the survival rate is 16.97% when the concentration is 64 μg/mL after 3 days. Conclusion The proliferation of glioma G422 cells can be inhibited by eholchieines, in a concentration and time-dependent manner.