特应性皮炎患者IL-4及IFN-γ的检测

Measurement of IL-4 and IFN-γ in Atopic Dermatitis

目的检测AD患者血清IL-4及IFN-γ水平,探讨AD的发病机理.方法收集符合Williams诊断标准的23例特应性皮炎(AD)患者血清,以酶联免疫吸附试验(ELISA)方法测定其血清IL-4及IFN-γ水平.以免疫荧光-酶技术测定血清总IgE抗体水平.结果AD患者的血清IL-4水平低于健康对照者的血清IL-4水平(P<0.01).AD患者与健康对照者之间血清IFN-γ水平无差异(P>0.05).AD患者总IgE抗体水平高于健康对照者.结论IFN-γ水平的相对升高可能在AD的慢性炎症中发挥作用.

Objective To measure of IL-4 and IFN- γ in atopic dermatitis. To analyse the pathogenesis of AD. Methods We collected 23 cases of patients with AD who meet the Williams criteria. The levels of total IgE in serum of 23 cases of AD and 19 healthy controls were measured with fluorescence-enzyme immunoassay. Their levels of IL-4 and IFN-γ in serum were measured with enzyme-linked immunosorbent assay （ELISA）. Results The level of serum total IgE antibody of AD was significantly higher than that in healthy controls. The level of IL-4 in serum was significantly lower in patients with AD than in healthy controls. There was difference in the level of IFN-γ in serum between the groups. Conclusion The higher ratio of IFN- γ / IL-4 may play a role in the chronic inflammation of AD. （The chronic inflammation of AD may be precipitated not by enhancing IFN- γ production, but by reducing IL-4 secretion.）