MN/CAⅨ基因在宫颈上皮内瘤变及宫颈鳞癌中的表达

Expression of MN/CAⅨ Gene in Cervical Intraepithelial Neoplasia and Cervical Squamous Cell Cancer

目的:探讨MN/CAⅨ基因在不同等级宫颈病变中的表达情况,其在宫颈病变的病情检测中的价值。方法:以我院妇科2005年6月至2005年10月阴道镜指引下活检确诊的患者86例为研究对象,其中慢性宫颈炎25例,CIN1级21例,CIN2级15例,CIN3级12例,宫颈鳞癌13例:取宫颈脱落细胞提取总RNA,行逆转录—多聚酶链反应(RT-PCR)观察MN/CAⅨ基因表达情况。结果:不同宫颈病变脱落细胞的MN/CAⅨ基因表达情况:宫颈炎12%,CIN1级76.19%,CIN2级86.67%,CIN3级91.67%,宫颈鳞癌92.31%,扩增出MN/CAⅨcDNA目的片段。CIN及宫颈磷癌患者的MN/CAⅨ基因表达率明显高于宫颈炎性病变者(P<0.001)。结论:MN/CAⅨ基因在CIN与宫颈癌中高表达,可用以鉴别宫颈瘤性病变及宫颈癌。宫颈脱落细胞MN/CAⅨ基因检测可以作为CIN病情监测的辅助指标。MN/CAⅨ基因可能成为一种有潜力的预测宫颈瘤性病变的肿瘤标记物。

Objective： To study the expression of MN/CAⅨ gene in the mutiple stages of cervical cancer, and evaluate the role of detecting the gene for the genesis of cervical cancer. Methods： The cervical scraping cells and biopsy tissue were collected for 86 patients, which consisting 25 cases of cervicitis , 21 cases of CIN1, 15 cases of CIN2,12 cases of CIN3, and 13 cases of squamous carcinoma of cervix. The presence of MN/CAⅨ was detected in all the cervical scraping cells using two-step RT-PCR. Resuits： The rates of the corresponding bands detected by RT-PCR in cervical scraping cells were 12% ,76.19%, 86.67%, 91. 67%, 92.31% in cervicitis , CIN1, CIN2, CIN3, and cervical cancer respectively . X^2 test indicated that there was significant difference between benign cervicitis and CIN / cervical cancer in the expression rate of MN （ P 〈 0.001 ）. The positive expression rate of MN increased gradually with the grades of CIN increasing, but there were no significant difference among them（ P 〉 0.05）. Conclusion： MN/CAⅨ was highly expressed in CIN and cervical cancer. It is a useful marker for the differentiation between cervical neoplasia , cervical cancer and benign cervicitis MN/CAⅨ expression may correlate with progress of cervical neoplasia. The expression of the MN/CAⅨ detected in cervical scraping cells may be used as a potential biomarker for momitoring cervical neoplasia.