KDR蛋白在卵巢浆液性肿瘤组织中的表达及临床意义

The Expression of KDR Protein in Ovarian Serous Carcinoma and Its Clinical Relevance

目的:探讨卵巢浆液性肿瘤(OSC)与正常卵巢组织中血管内皮生长因子受体(KDR、蛋白)的表达情况及临床意义。方法:利用Western Blotting方法对38例OSC、14例卵巢交界性浆液性瘤、11例卵巢良性浆液性瘤以及10例正常卵巢组织中KDR蛋白进行检测。结果:OSC组织中KDR蛋白表达的阳性率(89.5%)高于卵巢交界性浆液性瘤组织(64.3%)(P<0.05)。OSC组织中KDR蛋白表达的相对含量(141.10±87.31)明显高于卵巢交界性浆液性瘤(65.60±11.61)组织(P<0.005)。KDR蛋白在OSC和卵巢交界性浆液性瘤组织中表达的阳性率和相对含量均高于卵巢良性浆液性瘤(18.2%,42.62±12.66)及正常卵巢(10.0%,37.72±12.96)组织(P<0.05)。手术病理分期Ⅲ期+Ⅳ期OSC组织KDR蛋白表达的阳性率(96.2%)和相对含量(181.96±96.37)高于Ⅰ期+Ⅱ期(75.0%,112.37±59.28)OSC组织(P<0.05)。组织学Ⅲ级OSC组织KDR蛋白表达的阳性率(95.5%)和相对含量(171.76±91.66)高于Ⅰ级(4/6,87.96±35.59),有统计学意义(P<0.05)。有淋巴结转移的OSC组织中KDR蛋白表达的阳性率(96.2%)和相对含量(219.25±92.4)与无淋巴结转移的OSC组织(75.0%,108.43±30.45)相比较,有统计学意义(P<0.05)。有腹水的OSC组织中KDR蛋白表达的阳性率(83.3%)及相对含量(139.94±83.94)与无腹水的OSC组织(85.7%,167.07±93.66)相比较,无统计学意义(P>0.05)。结论:卵巢浆液性肿瘤的发生、发展与KDR蛋白的过高表达密切相关。KDR蛋白表达与卵巢浆液性癌生物学行为相关。

Objective： To study the expression of KDR protein in ovarian serous carcinoma, and compared with normal ovary tissue to find their clinical relevance. Methods： The technique of Western Blot was used to detect the expression of KDR protein in 38 cases of ovarian serous carcinoma, 14 cases of ovarian borderline serous tumors, 11 cases of ovarian benign serous tumors and 10 cases of normal ovary tissues. Results： The positive rate （89.5%） and relative content （ 141.10 ± 87.31 ） of KDR protein expression in the ovarian serous carcinoma were statistically higher than that in the ovarian borderline serous tumors（ 64. 3% ,65.60 ± 11. 61 ）. The positive rate and relative content in ovarian serous carcinoma and ovarian borderline serous tumors were statistically higher than that in ovarian benign serous tumors （ 18.2% ,42.62 ± 12.66） and normal ovary tissues（ 10.0% ,37.72 ± 12.96）. The positive rate and relative content of KDR protein expression in operative stage Ⅲ＋ Ⅳ （96.2% ,181.96 ±96.37）were statistically higher than that in stage Ⅰ ＋Ⅱ（75.0%, 112.37 ± 59.28 ）. The positive rate and relative content of KDR protein expression in pathological grade Ⅲ（95.5%, 171.76 ± 91.66） were statistically higher than that in grade Ⅰ（4/6,87.96 ±35.59）. The positive rate and relative content in ovarian serous carcinoma with lymph nodes metastasis （96.2%, 219.25 ± 92. 4） were statistically higher than that without lymph nodes metastasis （75.0%, 108.43 ± 30.45 ）. The positive rate and relative content had no significant differences between the patients with or without ascites. Conclusion： The occurrence and develonment of ovarian serous carcinoma and ovarian borderline serous tumors are correlated to the overexpression of KDR protein closely. The relationship between KDR protein expression and the operative stages, pathological grades and lymph node metastasis of ovarian serous carcinoma are existed.