端粒酶和Ki-67在卵巢上皮性肿瘤中的表达

The Study of the Expression and correlation of Telomerase and Ki-67 in Ovarian Epithelial Tumors

目的:研究端粒酶的催化亚基hTERT(human telomerase reverse transcriptase)和Ki-67在卵巢上皮性肿瘤中的表达情况及其意义。方法:采用原位杂交方法检测卵巢恶性肿瘤35例、临界恶性肿瘤(Low-Malignant-Po-tential tumor,LMP)8例和良性上皮源性肿瘤20例hTERT基因的表达,同时用免疫组化检测与细胞增殖相关的核抗原Ki-67的表达。结果:63例卵巢上皮性肿瘤中,良性肿瘤hTERT基因表达阳性率5%(1/20),临界恶性肿瘤阳性率38%(3/8),恶性肿瘤阳性率74%(26/35),良性、临界恶性和恶性肿瘤间hTERT的表达存在着显著性差异(P<0.05)。Ki-67阳性分布为:良性肿瘤均为阴性表达,临界恶性肿瘤表达率25%(2/8),恶性肿瘤表达率49%(17/35),良性、低度恶性和恶性肿瘤间Ki-67核抗原的表达存在着显著性差异(P<0.01)。结论:原位杂交检测hTERT基因的表达对于鉴别诊断良恶性卵巢上皮性肿瘤有参考价值;hTERT表达的上调可能在卵巢上皮性肿瘤的进展中起重要作用;hTERT基因表达与Ki-67核抗原表达有弱正相关性(r=0.274,P<0.05)。

Objective： To study the expression of human telomerase reverse transcriptase （hTERT） and Ki -67 in ovarian epithelial tumors. Methods： In situ hybridization technique were used to measure the level of hTERT mRNA in malignant ovarian epithelial tumors （ n = 35 ）, ovarian low-malignant-potential tumors （ n = 8） and benign ovarian diseases （ n = 20 ）, and immunohistochemistry was used to measure the expression of Ki-67. Results： A statistically significant difference was observed in the expression of hTERT messenger RNA （mRNA） in three groups. In comparison with benign （5%） and low-malignant-potential （38%） tumor, much higher frequency of telomerase activity was observed in malignant tumor（74% ）. The Ki-67 labeling index was positive in 25% （2/8） low-malignant-potential cases, and 49% （17/35） malignant cases. The Ki-67 labeling index was negative in benign ovarian tumors （0/20）. This result was significantly different from that of low-malignant-potential cases （ P = 0.02） and malignant cases （ P = 0.00）. Conclusion： Our results demonstrated that hTERT expression may be a good marker in diagnosis of malignant ovarian epithelial tumors and its up-regulation may play an important role in the progression of ovarian epithelial tumors. The Ki-67 expression level weakly related to the intensity of hTERT mRNA expression （ r = 0. 274, P = 0.030）.