玉米籽粒花色苷色素提取方法研究

Condition Optimization for Anthocyanin Extraction from Maize Grain

以黑包公鲜玉米为原料,通过单因素试验,探索了甲醇提取玉米籽粒花色苷色素的条件:玉米籽粒鲜样,1%盐酸甲醇,浸提2次,每次1.5 h,提取温度50℃,6 000 r/min离心10 min,离心温度25℃。

The conditions for extracting Anthocyanin from black maize with HCl methanol was optimized by monofactorial experiments. The optimum extraction conditions were as follows： fresh sample, 1% HCl methanol, two 1.5 - hour extractions at 50℃ and 10 - minute centrifugation at 6 000 r/min and 25℃.