摘要
通过对传统的比浊法中菌体的培养条件以及测定方法的改进,确定菌液的接种量为0.5mL,培养时间是24.5h,菌液的OD值调节到0.8,以及待测样品稀释倍数。成功地克服了菌在测定过程中不稳定、重复性差的问题,准确地测定了溶菌酶生产过程中各个步骤的样品活力,体现了生产过程的基本变化趋势,为溶菌酶生产过程提供了可靠的检测依据。
In this report, based on the improvement of cell culture conditions and the methods, the instability and poor reproducibility of the cell existing in traditional turbidimetrlc are overcomed successfully. The vitalities of the samples in the various steps of the production process are determinated accurately to show the basic trend of the production process, and provide a reliable detection basis for production process.
出处
《食品科技》
CAS
北大核心
2008年第11期10-13,共4页
Food Science and Technology
关键词
溶菌酶
跟踪监测
溶壁微球菌
重复性差
Lysozyme
tracking
dissolved wall micrococcus
poor repeatability
