胃癌术前、术后外周血与术中瘤旁血中血管内皮生长因子C的检测及其临床意义

Serous concentration of VEGF-C in gastric cancer patients and it＇ s clinical significance

目的检测胃癌患者术前、术后外周血和术中瘤旁血中血管内皮生长因子C（VRGF—C）浓度，探讨其临床意义及作为胃癌临床评估指标的可能性。方法接受胃癌根治术的患者108例，分别取术前外周静脉血、术中瘤旁静脉血、术后7d外周静脉血，酶联免疫吸附试验（ELISA）定量试剂盒检测VEGF-C血清浓度，比较各时段的浓度值，以36例非肿瘤病例的术前外周血浓度为对照。分析VEGF—C血清浓度与胃癌临床病理参数的关系。结果胃癌术前外周血、术中瘤旁血与术后7d外周血VEGF—C浓度（ng／L）分别为（4819．34±2112．65）、（5976．24±2222．75）、（2955．73±948．49）ng／L，瘤旁血浓度显著高于外周血，术后7dVEGF—C浓度较术前显著下降，三者之间差异有统计学意义（P〈0．01）。非肿瘤患者VEGF—C术前外周血清浓度为（2509．61±730．97）ng／L，显著低于胃癌患者术前外周血清浓度（P〈0．05）。胃癌术前外周血、术中瘤旁血VEGF-C血清浓度与肿瘤浸润深度、TNM分期、淋巴结转移状况显著正相关（P〈0．05）。术后7dVEGF-C外周血清浓度与各临床病理参数无显著相关性（P〉0．05）。结论胃癌患者术前VEGF—C外周血清浓度检测可以作为胃癌的筛查和诊断指标，瘤旁血VEGF—C浓度与肿瘤进展相关，根治术后VEGF—C浓度检测可能成为监测肿瘤复发的指标之一。

Objective To investigate the changes of serous concentration of VEGF-C in gastric cancer patients and it＇ s clinical significance. Methods In 108 gastric cancer patients treated by radical resection,the blood samples were taken from peripheral vein before operation, peritumor vein during operation and peripheral vein 7 d after operation respectively. Then the serous concentraion of VEGF-C was detected by ELISA kit and the concentrations on different times were compared. 36 nontumorous cases received selective operation were used as control. In gastric patients, the relationships between the concentration of VEGF-C and clinical pathological parameters were analyzed. Results Among the patients with gastric cancer, the concentrion of VEGF-C in intraoperative peritumor blood was （5976.24 ± 2222.75 ） ng/L,significantly higher than （4819. 34 ± 2112. 65 ） ng/L in preoperative peripheral blood and （2955.73 ± 948.49） ng/L in postoperative peripheral blood respectively （ P 〈 0.01 ）. The concentration of VEGF-C in postoperative peripheral blood significantly decreased compared with postoperative level （ P 〈 0.01 ）. The concentration of VEGF-C in preoperative peripheral blood in nontumor cases was （2509. 61 ± 730. 970） ng/L, significantly lower than that in gastric cancer cases （P 〈 0.05 ）. The concentrations of VEGF-C in preoperative peripheral and intraoperative peritumor blood were significantly correlated with infiltration depth, TNM staging,lymphatic metastasis （ P 〈 0.05 ）. Conclusion Detection of VEGF-C level in preoperative peripheral blood can become one of the screening and diagnostic measurements in gastric cancer, and can be used as a risk factor for recurrence after radical resection.