低氧对小鼠骨髓间质干细胞增殖及黏附的影响

Effects of low oxygen tension on the proliferation and adhesion of mouse MSCs

目的观察低氧环境对小鼠骨髓间充质干细胞（MSCs）增殖、黏附及分泌的影响。方法采用细胞增殖实验、细胞黏附实验及Western blot分别观察小鼠MSCs在低氧（6％O2）和常氧（21％O2）的增殖、黏附，及纤连蛋白（FN）和玻璃粘连蛋白（VN）的表达。结果低氧组10d内培养即可扩增到（1．20±0．19）×10^5细胞／孔，明显高于常氧组（0．65±0．08）×10^5细胞／孔（P〈0．05）；低氧组24h细胞黏附率（88．64±1．4）％高于常氧组（60．83±1．6）％（P〈0．05），同时FN和VN的蛋白表达显著增加。结论低氧作为体外的一种可控制因素，调节MSCs的增殖及黏附功能，对于MSCs的组织工程的应用具有一定的意义。

Objective To investigate the effects of low oxygen tension on the proliferation, adhesion and secretion of mouse mesenchymal stem cells （MSCs）. Methods Proliferation test,adhesion assay, and Western blot were performed to observe proliferation, adhesion and the protein expression of fibroneetin （FN） and vitronectin （VN） of mouse MSCs cultured in 6% O2 and 21% O2. Results Compared to 21% O2, （1.2 ± 0.19） × 10^5 cells/well could be obtained after 10 days culture in 6% 02 （P 〈 0. 05 ）. Ratio of 24 h adhesion in 6% O2 group [ （88.64 ± 1.4 ）% ] was significantly higher than that in 21% O2 group [ （60.83 ± 1.6） % ] （ P 〈 0.05 ）. The protein expression of FN and VN were also higher in 6% 02 group. Conclusion Low oxygen tension,as an in vitro factor which could be controlled,can regulate MSCs in proliferation, adhesion and extracellular matrix secretion, which plays a role in the application of MSCs in tissue engineering.