摘要
为了初步研究蛇足石杉内生真菌g5发酵产物的乙酰胆碱酯酶(AChE)抑制动力学以及对该株真菌做初步形态学鉴定,采用DTNB显色法检测g5发酵液醇提物抑制AChE活性的效果,并采用玻片培养法进行真菌鉴定。结果发现g5菌株属于半知菌目丛梗孢科青霉属;其发酵液醇提物对AChE的抑制作用表现为混合竞争型可逆抑制,其对游离酶的抑制常数KI与对酶底物络合物的抑制常数KIS分别为0.0789 mL和1.1352 mL,因此g5菌株代谢产物可作为开发AChE抑制剂类药物的潜在资源。
It was about the initially research in the kinetics of inhibiting acetylcholinesterase(AChE)of endophytic fungi g5 which Isolated from Huperzia serrata.In addition,morphologic determination was done with this fungus.The effect of EtOH extraction from g5 fermentative product on the inhibiting AChE was detected by coloration of DTNB method,and this fungus was determinated by slide culture.The result is that g5 belongs to Penicillium rugulosum.According to Initially analysis of enzyme kinetics,it was showed that g5 was admixed competitive reversible inhibitory type,and the inhibit-constant KI against free E and KIS against comoles compound of ES is 0.0789 mL and 1.1352 mL,respectively.The metabolic product of g5 can be developed as potential drugs which is not only conspicuous but also reversible inhibiting AChE in vitro.
出处
《微生物学通报》
CAS
CSCD
北大核心
2008年第11期1764-1768,共5页
Microbiology China
基金
中国科学院知识创新工程重大项目子课题(No.KSCX1-SW-11-6)
关键词
乙酰胆碱酯酶
蛇足石杉
内生真菌
Acetylcholinesterase,Huperzia serrata,Endophytic fungi