大孔吸附树脂对丁香叶中丁香苦苷的富集工艺研究

Enrichment process of syringopicroside in Folium Syringae Oblatae with macroporous resin

目的研究大孔吸附树脂富集丁香苦苷类成分的工艺条件及参数。方法以丁香苦苷为考察指标,建立HPLC分析方法,通过考察6种大孔吸附树脂对丁香苦苷的吸附分离性能,筛选出AB-8树脂作为分离纯化丁香苦苷类成分的介质,并对AB-8树脂的吸附性能进行研究,考察富集丁香苦苷类成分的最佳工艺条件。结果丁香叶经水提醇沉浓缩后,以AB-8型大孔树脂为吸附剂,4BV的水洗脱出去杂质后,收集5BV50%乙醇洗脱液为最佳工艺条件。结论大孔吸附树脂分离工艺可作为富集丁香苦苷类成分的有效方法,并为丁香叶药材的质量控制和新型给药系统的研究奠定基础。

Objective To study the optimal conditions and parameters for the enrichment process of syringopicroside in Folium Syringae Oblatae with macroporous resin. Methods With the enrichment degree of syringopicroside as an index, the analytical method of HPLC was developed, AB-8 resin was selected as the medium for purification of syringopicroside on the basis of comparing six kinds of macroporous resin＇s adsorption and separation performances, and the optimal conditions of the enrichment process were investigated. Results The optimal conditions were that the extract of Folium Syringae Oblatae was extracted by water and condensed by alcohol precipitation taking AB-8 resin column as adsorbent, the resin was washed by 4 BV of distilled water to remove impurity and 5 BV of 50% ethanol to elute syringae. Conclusion The process is an effective method to enrich syringopieroside and provide a way to research new medicine delivery system.