空心莲子草的高效毛细管电泳指纹图谱研究

HPCE Fingerprint of Alternanthera philoxerodes

目的利用高效毛细管电泳技术建立空心莲子草药材的指纹图谱。方法采用60cm×75μm毛细管柱,以50mmol/L硼砂溶液-50mmol/L磷酸盐溶液为缓冲液,运行电压为20kV,检测波长220nm,柱温25℃。结果建立的指纹图谱中共有18个共有峰,且方法学考察符合规定的标准。结论该法准确简便,可作为控制空心莲子草药材内在质量的有效手段。

Objective To investigate the high performance capillary electrophoresis （HPCE） fingerprint of Alternanthera philogcerodes. Methods Separation was performed on a 60 cm× 75μm uncoaled capillary with 50 mmol/L borate solution-50 mmol/L phosphate solution （pH 8.76） as HPCE buffer. The running voltage was 20 kV, the detection wavelength was 220 nm and temperature 25℃. Results Fingerprint was established with 18 common peaks. Conclusion The method is accurate, simple, and suitable to the quality control of A. philoxerodes.