变温培养对羊草胚性愈伤组织诱导率的影响

Effect of Variable Cultivating Temperature on Frequency of Embryogenic Callus Inducting from Leymus chinensis

目的:通过变温处理研究羊草种子休眠与愈伤组织诱导的关系。方法:以成熟羊草种子作为外植体,MS+2.0mg/L2,4-D为诱导培养基,分别采用26℃恒温和16℃/26℃变温(各12h)的方式培养,3w后统计诱导率。结果:恒温和变温培养均能诱导出愈伤组织细胞,变温培养羊草愈伤组织诱导率达到48.25%,较之恒温培养高出约1倍。结论:变温培养有助于羊草胚性愈伤组织的诱导。

Objective： Investigate the relation between dormancy and embryogenic callus induction of Leymus chinensis with variable cultivating temperature. Methods： Using seeds as explant and MS ＋ 2.0mg/L 2,4 - D as culture medium, callus induction was performed at 26℃ and 16℃/26℃ respectively and frequency was calculated after three weeks. Result： Callus can all be inducted with constant and variable temperatttre, but the later frequency （average 47.6% ） was two times of the former. Conclusion： Variable temperature is helpful to the embryogenic callus induction of Leymus chinensis.