花生胚小叶离体再生体系的优化研究

Studies on Optimization of the Regeneration Technique from Leaflet of Peanut

目的:研究培养基中植物激素的种类、浓度和基因型差异对花生胚小叶离体培养再生效率的影响。方法:以花生胚小叶为外值体,比较各处理间的芽丛诱导率、芽丛出苗率和植株再生率,确定每个品种对应的适宜培养基。结果:小花生品种丰花2号的适宜芽丛诱导培养基为MSB+3.0mg/LBA+0.7mg/LNAA,芽丛诱导率为81.49%,再生率为94.39%;适宜芽丛成苗培养基为MSB+2.5mg/LBA,芽丛出苗率为172.00%,再生率为122.10%。大花生品种丰花3号的适宜芽丛诱导培养基为MSB+4.5mg/LBA+0.2mg/LNAA,芽丛诱导率为72.33%,再生率为10.70%;适宜芽丛成苗培养基为MSB+5.0mg/LKIN,芽丛出苗率为33.33%,再生率为4.03%。结论:不同基因型之间的成苗芽丛百分率、芽丛出苗率和植株再生率差异显著。

Objective： The effects of plant hormone（including variety and deepness）and genotype on the regeneration of peanut（ Arachis hypogaea L. ） leaflet explants were investigated. Method： The rate of buds inducement,buds elongation and plant regeneration were compared among different disposal. The best culture medium for the two cuhivars were confirmed. Result： The suitable medium for the inducement of buds was MSB ＋ 3.0mg/L BA ＋ 0.7mg/L NAA for Fenghua 2, frequencies of buds inducement and regeneration were 81.49% and 94.39% respectively; the best media for the elongation of buds were MSB ＋ 2.5mg/L BA for Fcnghua 2, the rate of seeding in buds and regeneration were 172.00% and 122.10% respectively; MSB ＋ 4.5mg/L BA ＋ 0.2mg/L NAA was suitable inducement medium for Fenghua 3, frequencies of buds inducement and regeneration were 72.33% and 10.70% respectively;the best media for the elongation of buds were MSB ＋ 5.0mg/L KIN for Fenghua 3, the rate of seeding in buds and regeneration were 33.33% and 4.03% respectively. Conclusion： There were significant differences on bud differentiation and plant regeneration between the two genotypes.